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AAT Bioquest

FAQ

Posted on November 11, 2023
What are the advantages of digital PCR (dPCR)?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Tide Quencher DyesDigital PCR


Posted on August 29, 2023
How does a molecular beacon work?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes


Posted on August 14, 2023
Why are quenchers used in real time PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use one-step as opposed to two-step qRT-PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 12, 2023
What are the factors that cause fluorescence quenching?
Bioconjugation Förster Resonance Energy Transfer (FRET)Chemical ReagentsFluorescencePhysiological ProbesTide Quencher Dyes


Posted on June 2, 2022
What is the difference between Tide Quencher 2 (TQ2) and Tide Quencher 2WS (TQ2WS) products?
Tide Quencher Dyes Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingAntibody and Protein Labeling Chemical Reagents


Posted on January 26, 2021
How are oligonucleotides synthesized?
Tide Quencher DyesPeptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)Chemical Reagents